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- Title
Comprehensive autoantibody profiling in systemic autoimmunity by a highlysensitive multiplex protein array.
- Authors
Ai Kuzumi; Yuta Norimatsu; Matsuda, Kazuki M.; Chihiro Ono; Taishi Okumura; Emi Kogo; Naoki Goshima; Takemichi Fukasawa; Natsumi Fushida; Motoki Horii; Takashi Yamashita; Asako Yoshizaki-Ogawa; Kei Yamaguchi; Takashi Matsushita; Shinichi Sato; Ayumi Yoshizaki
- Abstract
Comprehensive autoantibody evaluation is essential for the management of autoimmune disorders. However, conventional methods suffer from poor sensitivity, low throughput, or limited availability. Here, using a proteome-wide human cDNA library, we developed a novel multiplex protein assay (autoantibody array assay; A-Cube) covering 65 antigens of 43 autoantibodies that are associated with systemic sclerosis (SSc) and polymyositis/dermatomyositis (PM/DM). The performance of A-Cube was validated against immunoprecipitation and established enzyme-linked immunosorbent assay. Further, through an evaluation of serum samples from 357 SSc and 172 PM/DM patients, A-Cube meticulously illustrated a diverse autoantibody landscape in these diseases. The wide coverage and high sensitivity of A-Cube also allowed the overlap and correlation analysis between multiple autoantibodies. Lastly, reviewing the cases with distinct autoantibody profiles by A-Cube underscored the importance of thorough autoantibody detection. Together, these data highlighted the utility of A-Cube as well as the clinical relevance of autoantibody profiles in SSc and PM/DM.
- Subjects
PROTEIN microarrays; AUTOANTIBODIES; AUTOIMMUNE diseases; AUTOIMMUNITY; ENZYME-linked immunosorbent assay; SYSTEMIC scleroderma
- Publication
Frontiers in Immunology, 2023, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2023.1255540