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- Title
Colonization of microflora in mice: mucosal defense against luminal bacteria.
- Authors
Fukushima, Kouhei; Sasaki, Iwao; Ogawa, Hitoshi; Naito, Hiroo; Funayama, Yuji; Matsuno, Seiki; Fukushima, K; Sasaki, I; Ogawa, H; Naito, H; Funayama, Y; Matsuno, S
- Abstract
To investigate the pathogenesis of inflammatory bowel disease, it is critical to develop a system that uses simple and reproducible models for analyzing the "normal" mucosal defense mechanism. In the present study, germ-free mice were conventionalized by the oral administration of microorganisms prepared from the feces of genetically identical mice. Histological assessment and mucin characterization of small intestine and colon were then carried out. Histological findings in the gut were site-dependent and clearly time-dependent. Acute inflammation was most evident in the cecum. The cecal mucosa exhibited hyperplastic changes in epithelial cells, infiltration of polymorphonuclear cells, crypt abscesses, and epithelial projections on the epithelial surface 7 days after conventionalization. Some of the changes were similar to those seen in human ulcerative colitis. The histological findings in the conventionalized mice were comparable to those in specific pathogen-free mice after 28 days. Mucin histochemistry revealed that bacterial colonization altered the number of rectal goblet cells and the mucin composition in a time-dependent fashion. Although this model shares only some characteristics of human inflammatory bowel disease, it is unique in demonstrating the acquisition of mucosal defense. Understanding of this process is critical for the elucidation of inflammatory bowel disease pathogenesis.
- Subjects
INFLAMMATORY bowel diseases; BACTERIA; MUCINS; SMALL intestine; COLON (Anatomy); PATHOLOGY; PROTEIN metabolism; COLON microbiology; ANIMAL experimentation; BACTERIAL growth; COMPARATIVE studies; INTESTINAL mucosa; LONGITUDINAL method; RESEARCH methodology; MEDICAL cooperation; MICE; MICROBIOLOGICAL techniques; RESEARCH; TIME; EVALUATION research; SEVERITY of illness index; PERIODIC acid-Schiff reaction
- Publication
Journal of Gastroenterology, 1999, Vol 34, Issue 1, p54
- ISSN
0944-1174
- Publication type
journal article
- DOI
10.1007/s005350050216