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- Title
IMPROVED REFERENCE GENOME FOR CYCLOTELLA CRYPTICA CCMP332, A MODEL FOR CELL WALL MORPHOGENESIS, SALINITY ADAPTATION, AND LIPID PRODUCTION IN DIATOMS (BACILLARIOPHYTA).
- Authors
Roberts, Wade R.; Downey, Kala M.; Ruck, Elizabeth C.; Traller, Jesse C.; Alverson, Andrew J.
- Abstract
he diatom, Cyclotella cryptica, is a well-established model species for physiological studies and biotechnology applications of diatoms. To further facilitate its use as a model diatom, we report an improved reference genome assembly and annotation for C. cryptica strain CCMP332. We used a combination of long- and short-read sequencing to assemble a high-quality and contaminant-free genome. The genome is 171 Mb in size and consists of 662 scaffolds with a scaffold N50 of 494 kb. This represents a 176-fold decrease in scaffold number and 41-fold increase in scaffold N50 compared to the previous assembly. The genome contains 21,250 predicted genes, 75% of which were assigned putative functions. Repetitive DNA comprises 59% of the genome, and an improved classification of repetitive elements indicated that a historically steady accumulation of transposable elements has contributed to the relatively large size of the C. cryptica genome. The high-quality C. cryptica genome will serve as a valuable reference for ecological, genetic, and biotechnology studies of diatoms.
- Subjects
GENOMES; PHAEODACTYLUM tricornutum; HORIZONTAL gene transfer; MORPHOGENESIS; DIATOMS; TRANSPOSONS; SALINITY; ALGAL biofuels
- Publication
G3: Genes | Genomes | Genetics, 2020, Vol 10, Issue 9, p1
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1534/g3.120.401408