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- Title
Optimizing cryopreservation of nasal polyp tissue for cellular functional studies and single-cell RNA sequencing.
- Authors
Sohail, Aaqib; Baloh, Carolyn H.; Hacker, Jonathan; Cho, Laura; Ryan, Tessa; Bergmark, Regan W.; Lee, Stella E.; Maxfield, Alice; Roditi, Rachel; Dwyer, Daniel F.; Buchheit, Kathleen M.; Laidlaw, Tanya M.
- Abstract
This research note explores the optimization of cryopreservation methods for nasal polyp tissue to enable cellular functional studies and single-cell RNA sequencing. The study compares different cryopreservation methods and assesses their impact on cell viability, immune cell subset composition, and gene expression profiles. The findings suggest that the choice of cryopreservation method should be tailored to the specific goals and cell types of the research project. The study aims to enhance methodology for future clinical trials and tissue biobanks. The article also provides information on quality control parameters and the mast cell transcriptome. The authors disclose no conflicts of interest.
- Subjects
CRYOPRESERVATION of cells; NASAL polyps; RNA sequencing; PLASMA cells; PARANASAL sinus diseases; TISSUES
- Publication
International Forum of Allergy & Rhinology, 2024, Vol 14, Issue 5, p972
- ISSN
2042-6976
- Publication type
Article
- DOI
10.1002/alr.23275