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- Title
Activity of different defense related antioxidative biochemical compound upon exogenous application of different elicitors on maize against Helminthosporium maydis Y. Nisik & C. Miyake.
- Authors
Kumar, Chandan; Chand, Phool; Choudhary, C. S.; Maurya, Shivam; Kumar, Adesh; Priya, Shruti
- Abstract
Maydis leaf blight (MLB) caused by Helminthosporium maydis Y. Nisik. & C. Miyake is prevalent in almost all maize growing areas and is a major limiting factor in increasing yield. Knowing about the metabolic pathways that results in accumulation of enzymatic and non-enzymatic antioxidative biochemical compounds which constitutes a second line of defense, an in vitro experiment was conducted. Reactive oxygen species produced during stresses is one of the most instant defense reactions to arrest plant pathogen at the site of infection. Oxidative burst results in the production of ROS, mainly H2O2 and superoxide at the invading site. Production of secondary metabolites and induction of ISR and SAR mediate biochemical defenses in plant. In vitro study to observe the effect of different elicitors applied exogenously against H. maydis by investigating the biochemical parameters viz., Total phenols, Proline, Hydrogen peroxide (H2O2) and, enzymatic anti-oxidative biochemicals viz., Superoxide dismutase (SOD), Polyphenol oxidase (PPO), Peroxidase (POD) and, Phenylalanine ammoniase (PAL) activity is conducted. The activity of different biochemical parameters was observed on the 0th day (before inoculation), 3rd day, 6th day and 9th day of inoculation with the help of spectrophotometer. Accumulation of H2O2 concentration was recorded maximum (815.61 µmol g−1FW) at the 9th day after inoculation in 2,6-dichloroisonicotinic acid (INA)-treated plants. INA-treated plants showed highest proline accumulation (43.39 µmol g−1FW) and phenolics content (560.92 µg g−1FW) at the 6th and 3rd day after inoculation of the pathogen, respectively. In biochemical studies for elucidation of defence induction, biochemical markers reached maximum on the 3rd day after pathogen inoculation, and INA treatments were found most effective.
- Subjects
POLYPHENOL oxidase; SUPEROXIDE dismutase; METABOLITES; REACTIVE oxygen species; PHYTOPATHOGENIC microorganisms; PHENYLALANINE ammonia lyase
- Publication
Cereal Research Communications, 2024, Vol 52, Issue 2, p721
- ISSN
0133-3720
- Publication type
Article
- DOI
10.1007/s42976-023-00408-z