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- Title
Direct labeling of nucleosides with 3-thiazolylcoumarin fluorescent dyes.
- Authors
Kuziv, Ia. B.; Dubey, I. Ya.
- Abstract
Aim. Preparation and study of nucleosides labeled with coumarin-based fluorophores without preliminary functionalization. Methods. Organic synthesis, absorption and fluorescence spectroscopy. Results. Direct labeling of 2'-deoxynucleosides with carboxy-modified 3-thiazolylcoumarins was performed. Coumarin conjugates were obtained by the reacting of active oxybenzotriazole esters of the dyes with nucleoside 5'-hydroxyl in the presence of a base, and with cytosine amino group. Their optical properties in methanol and phosphate buffer were studied. Conclusion. N- and O-acylation of pyrimidine nucleosides with coumarin derivatives allowed to obtain conjugates with bright blue emission.
- Subjects
FLUORESCENT dyes; PYRIMIDINE nucleosides; LABELS; AMINO group; NUCLEOSIDES; COUMARINS; BENZOTRIAZOLE derivatives
- Publication
Biopolymers & Cell, 2020, Vol 36, Issue 1, p36
- ISSN
0233-7657
- Publication type
Article
- DOI
10.7124/bc.000A1F