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- Title
Enhanced Production of Malic Acid by Co-localization of Phosphoenolpyruvate Carboxylase and Malate Dehydrogenase Using Synthetic Protein Scaffold in Escherichia coli.
- Authors
Somasundaram, Sivachandiran; Jeong, Jaehoon; Irisappan, Ganesh; Kim, Tae Wan; Hong, Soon Ho
- Abstract
To produce malic acid from non-oxidative pathway route in Escherichia coli using two key enzymes and synthetic scaffold complex. E. coli was engineered to produce malic acid from glucose by co-localization of two key enzymes phosphoenolpyruvate carboxylase (Ppc) and malate dehydrogenase (MdhA) with synthetic scaffold complex. Scaffold plasmid has produced the maximum concentration of 3.51 g/L malic acid from 10 g/L glucose in 48 h of culture. pH 5.5 and temperature 30°C were optimum for malic acid production without any engineering of competing metabolic pathways. E. coli mutant strains and different concentrations of glucose also tested. When 50 g/L glucose was used as substrate, 20.4 g/L of malic acid was produced.
- Subjects
MALATE dehydrogenase; SYNTHETIC proteins; MALIC acid; SCAFFOLD proteins; ESCHERICHIA coli; ACETYLCOENZYME A
- Publication
Biotechnology & Bioprocess Engineering, 2020, Vol 25, Issue 1, p39
- ISSN
1226-8372
- Publication type
Article
- DOI
10.1007/s12257-019-0269-1