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- Title
Two-dimensional electrophoresis study of in vitro pellicle formation and dental caries susceptibility.
- Authors
Vitorino, Rui; De Morais Guedes, Sofia; Ferreira, Rita; Lobo, Maria João C.; Duarte, José; Ferrer‐Correia, António J.; Tomer, Kenneth B.; Domingues, Pedro M.; Amado, Francisco M. L.
- Abstract
In the present study, a proteomic approach was applied to evaluate the influence of salivary protein composition on in vitro dental pellicle formation and its possible correlation with dental caries. Whole saliva, collected from caries-free and caries-susceptible subjects, was analyzed by two-dimensional electrophoresis, and protein spots were identified by mass spectrometry. Data analysis of salivary protein composition showed a statistically significant correlation between the quantity of acidic proline-rich proteins (PRPs) , lipocalin, cystatin SN and cystatin S, and samples from the caries-free group of subjects [decayed, missing or filled teeth (DMFT) = 0]. Samples from subjects with a high DMFT index appear to be correlated with high levels of amylase, immunoglobulin A, and lactoferrin. In vitro pellicle-composition experiments showed the same correlations found for whole saliva. As cystatins are known physiological inhibitors of cathepsins, the higher quantities of lipocalin, and cystatins S and SN found in the samples from the caries-free subjects suggest that inhibition of proteolytic events on other salivary proteins may indirectly provide tooth protection. The correlation between higher levels of the phosphorylated acidic PRPs 1/2 with samples from the caries-free group also suggests a protective role for these proteins.
- Subjects
ELECTROPHORESIS; DENTAL caries; MASS spectrometry; AMYLASES; SALIVA
- Publication
European Journal of Oral Sciences, 2006, Vol 114, Issue 2, p147
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1111/j.1600-0722.2006.00328.x