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- Title
Analysis of HLA-G Polymorphisms in Couples with Implantation Failure.
- Authors
Nardi, Fabiola da Silva; Slowik, Renata; Wowk, Pryscilla Fanini; da Silva, José Samuel; Gelmini, Geórgia Fernanda; Michelon, Tatiana Ferreira; Neumann, Jorge; Bicalho, Maria da Graça
- Abstract
Problem HLA- G expression is related as an immune modulator of fetal-maternal tolerance, and its levels was correlated with pregnancy outcome. In a case-control study, we investigate the association between the genetic variability of the HLA-G gene and serum levels of soluble HLA-G in cases of embryo implantation failure. Method of Study Forty couples with at least two unsuccessful fresh embryo transfers (implantation failure; IF) and 83 fertile couples with at least two successful pregnancies was genotyped by sequencing-based typing. HLA- G alleles were defined by nucleotide sequence variations at exon 2, 3, and 4, and the quantification of soluble HLA- G (s HLA- G) was performed by ELISA. Results There was a significant difference between the HLA- G allelic distributions between IF couples and the control couples. The HLA-G*01:03:01 allele was increased in the IF couples. There were no significant differences in the serum levels of s HLA- G in the IF and control groups. Conclusion The results suggest that the distribution of HLA- G products may play a significant role in the modulation of maternal-fetal immune response.
- Subjects
HLA histocompatibility antigens; GENETIC polymorphisms; IMMUNOREGULATION; HUMAN embryo transfer; IMMUNOLOGICAL aspects of pregnancy; HUMAN genetic variation; ENZYME-linked immunosorbent assay
- Publication
American Journal of Reproductive Immunology, 2012, Vol 68, Issue 6, p507
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12001