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- Title
Characterization and quantitation of fluorescent Gag virus-like particles.
- Authors
Gutiérrez-Granados, Sonia; Cervera, Laura; Gòdia, Francesc; Mercedes Segura, María
- Abstract
The article presents a study which discusses characterization and quantitation of fluorescent Gag virus-like particles. Topics discussed include generation of fluorescent virus-like particles (VLPs) carried by transient transfection of human embryonic kidney 293 (HEK 293) suspension cells, VLP budding visualized by electron microscopy and confocal fluorescence microscopy, and fluorescently tagged Gag VLPs easily generated by expressing Gag as a fusion construct with green fluorescent protein.
- Subjects
GAG proteins; VIRUS-like particles; HUMAN embryonic stem cells; KIDNEY cell culture; ELECTRON microscopy; FLUORESCENT proteins
- Publication
BMC Proceedings, 2013, p1
- ISSN
1753-6561
- Publication type
Article
- DOI
10.1186/1753-6561-7-S6-P62