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- Title
Utilização da serologia na identificação de Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae).
- Authors
de Mello Costa, Fabiano; da Silva Loffredo, Ana Paula; Onody, Helena Caroline; Sousa-Silva, Carlos Roberto
- Abstract
Using the serological technique to identify Ascia monuste orseis (Latreille, 1819) (Lepidoptera: Pieridae). This study aimed to obtain specific antiserum for Ascia monuste orseis (Latreille, 1819), with the aim of identifying the predators associated with this insect. The preparation of specific antiserum was performed by immunizing a rabbit via a lymph nodule injection containing antigen obtained from the maceration of 4th and 5th instars of A. monuste orseis caterpillars. Serological homologous tests performed using double diffusion in agar gel were positive 21 days after the first antigen inoculation. Eggs, 1th, 2th and 3th instar caterpillars, pupae and adults of A. monuste orseis also reacted positively to the obtained antiserum.
- Subjects
LIFE sciences research; ASCIA monuste; ASCIA; LEPIDOPTERA; INSECTS; IMMUNOSPECIFICITY; ANTIBODY diversity; VACCINATION; IMMUNOLOGY
- Publication
Acta Scientiarum: Biological Sciences, 2009, Vol 31, Issue 2, p149
- ISSN
1679-9283
- Publication type
Article
- DOI
10.4025/actascibiolsci.v31i2.515