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- Title
Expression of ephrin-A2 in the superior colliculus and EphA5 in the retina following optic nerve section in adult rat.
- Authors
Rodger, J.; Lindsey, K. A.; Leaver, S. G.; King, C. E.; Dunlop, S. A.; Beazley, L. D.
- Abstract
Abstract The vertebrate retina projects topographically to visual brain centres. In the developing visual system, gradients of ephrins and Eph receptors play a role in defining topography. At maturity, ephrins but not Ephs are downregulated. Here we show that optic nerve section in adult rat differentially regulates the expression of ephrin-A2 in the superior colliculus (SC) and of EphA5 in the retina. Expression was quantified immunohistochemically; ephrin-A2 levels were also estimated by semiquantitative reverse transcriptase polymerase chain reaction. In the normal SC, ephrin-A2 was expressed at low levels. At 1 month, levels of protein and of mRNA were upregulated across the contralateral SC giving rise to an increasing rostro-caudal gradient. At 6 months, levels had fallen but a gradient remained. In the retina of normal animals, EphA5 was expressed as an increasing naso-temporal gradient. By 1 month, expression was decreased in far temporal retina, resulting in a uniform expression across the naso-temporal axis. We suggest that denervation-induced plastic changes within the SC modify expression of these molecules.
- Subjects
CELL receptors; RETINA; POLYMERASE chain reaction; CENTRAL nervous system
- Publication
European Journal of Neuroscience, 2001, Vol 14, Issue 12, p1929
- ISSN
0953-816X
- Publication type
Article
- DOI
10.1046/j.0953-816X.2001.01822.x