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- Title
Accessory cell functions in mononuclear cell cultures from uremic patients.
- Authors
Ladefoged, J.; Langhoff, E.
- Abstract
Mononuclear cells (PBMC) were isolated from dialyzed patients and healthy control subjects. Lymphocyte responses to stimulation with optimal and suboptimal concentrations of lectin (PHA), or stimulation with T cell receptor antibody (Leu 4) were found decreased in the patient cultures. The separate and the combined effects of exogenous interleukin-1 (IL-1) and interleukin-2 (IL-2) were examined in PHA and Leu 4 stimulated cell cultures. Addition of IL-1 did not normalize the decreased proliferation response of the patient cultures. In contrast, addition of IL-2 alone clearly enhanced and almost normalized the response of patient cultures stimulated with suboptimal concentrations of PHA. The combined addition of IL-1 and IL-2 gave no evidence of an additive effect of IL-1 and IL-2. Cell cultures from uremic and normal HLA-identical relative were examined. Substitution of uremic adherent monocytes with normal adherent monocytes as accessory cells did not improve the uremic T cell responses to stimulation with PHA. Furthermore, uremic adherent cells did not suppress the normal T cell responses. These results suggest that uremic accessory cells support T cell activation and, in particular, do not suppress T cell responses. The effect of IL-2 in the present study as well as previous findings of decreased IL-2 production in patients cultures may indicate that uremia primarily influences the proliferation of T cells.
- Subjects
CELL physiology; ANTIGEN presenting cells; IMMUNOCOMPETENT cells; CELL culture; UREMIA; PATIENTS; LYMPHOCYTES; CELL proliferation; T cells
- Publication
Kidney International, 1990, Vol 37, Issue 1, p126
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1990.17