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- Title
Urinary excretion of C5b-9 reflects disease activity in passive Heymann nephritis.
- Authors
Pruchno, Charles J.; Burns, Mark W.; Schulze, Matthias; Johnson, Richard J.; Baker, Patricia J.; Couser, William G.
- Abstract
Passive Heymann nephritis (PEN) is a model of membranous nephropathy in rats in which glomerular injury is mediated by the terminal C5b-9 membrane attack complex of complement. This model has been shown to be associated with markedly elevated urinary excretion of C5b-9, compared to other experimental models of glomerulonephritis. To determine if urinary C5b-9 excretion could serve as an index of disease activity by correlating with the formation and quantity of glomerular subepithelial immune deposits in PHN, we measured urinary excretion of C5b-9 in PHN under several experimental conditions. In the heterologous phase a direct correlation was demonstrated between levels of urinary C5b-9 excretion and the amount of anti-Fx1A IgG deposited in glomeruli (r = 0.85). In the autologous phase, C5b-9 excretion correlated with the amount of deposit forming antibody present in the serum and resolved when antibody disappeared, despite persistence of glomerular deposits of antigen, antibody, C5b-9 and heavy proteinuria. Glomerular C3 deposits paralleled urinary C5b-9 excretion. Re-initiation of active deposit formation by a second injection of anti-Fx1A produced new C3 deposits and a marked rise in C5b-9 excretion. Finally, complete abrogation of deposit formation by transplanting PHN kidneys into normal recipients also halted C5b-9 excretion. Our findings demonstrate that urinary excretion of C5b-9 is a sensitive index of on-going immunologic disease activity in the PHN model of membranous nephropathy.
- Subjects
KIDNEY glomerulus; LABORATORY rats; EXCRETION; URINE; KIDNEY diseases; ANIMAL models in research
- Publication
Kidney International, 1989, Vol 36, Issue 1, p65
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1989.162