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- Title
Advantages of adipose tissue stem cells over CD34<sup>+</sup> mobilization to decrease hepatic fibrosis in Wistar rats.
- Authors
De Luna-Saldivar, Marcela M.; Marino-Martinez, Iván A.; Franco-Molina, Moisés A.; Rivera-Morales, Lydia G.; Alarcón-Galvánd, Gabriela; Cordero-Pérez, Paula; Rojas-Martínez, Augusto; Rodríguez-Padilla, Cristina; Muñoz-Espinosa, Linda E.
- Abstract
Introduction and Objectives: Chronic liver inflammation may lead to hepatic cirrhosis, limiting its regenerative capacity. The clinical standard of care is transplantation, although stem cell therapy may be an alternative option. The study aim was to induce endogenous hematopoietic stem cells (HSCs) with granulocyte colony stimulating factor (G-CSF) and/or intravenous administration of adipose tissue-derived mesenchymal stem cells (MSCs) to decrease hepatic fibrosis in an experimental model. Material and methods: A liver fibrosis model was developed with female Wistar rats via multiple intraperitoneal doses of carbon tetrachloride. Three rats were selected to confirm cirrhosis, and the rest were set into experimental groups to evaluate single and combined therapies of G-CSF-stimulated HSC mobilization and intravenous MSC administration. Results: Treatment with MSCs and G-CSF significantly improved alanine amino transferase levels, while treatment with G-CSF, MSCs, and G-CSF + MSCs decreased aspartate amino transferase levels. Hepatocyte growth factor (HGF) and interleukin 10 levels increased with MSC treatment. Transforming growth factor β levels were lower with MSC treatment. Interleukin 1β and tumor necrosis factor alpha levels decreased in all treated groups. Histopathology showed that MSCs and G-CSF reduced liver fibrosis from F4 to F2. Conclusions: MSC treatment improves liver function, decreases hepatic fibrosis, and plays an antiinflammatory role; it promotes HGF levels and increased proliferating cell nuclear antigen when followed by MSC treatment mobilization using G-CSF. When these therapies were combined, however, fibrosis improvement was less evident.
- Subjects
HEPATIC fibrosis; PROLIFERATING cell nuclear antigen; HEPATOCYTE growth factor; ADIPOSE tissues; STEM cells; HEPATIC veno-occlusive disease
- Publication
Annals of Hepatology: Official Journal of the Mexican Association of Hepatology, 2019, Vol 18, Issue 4, p620
- ISSN
1665-2681
- Publication type
Article
- DOI
10.1016/j.aohep.2018.12.005