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- Title
Filtration of native and glycated β<sub>2</sub>-microglobulin by charged and neutral dialysis membranes.
- Authors
Randoux, Christine; Gillery, Philippe; Georges, Nadine; Lavaud, Sylvie; Chanard, Jacques
- Abstract
Filtration of native and glycated β2-microglobulin by charged and neutral dialysis membranes. Background. It has been postulated that protein glycation and formation of advanced glycation end products (AGE) are among toxic factors in chronic uremia, whether the renal disease is of diabetic or nondiabetic origin. In this setting, AGE-modified β2-microglobulin (β2m) may favor dialysis β2m-related dialysis amyloidosis. Consequently, efficient removal of modified β2m by highly permeable dialysis membranes is as important as removal of native β2m to postpone the development of dialysis amyloidosis. Methods. To define the role of dialysis membrane surface electronegativity on plasma protein transfer, an in vitro model was used to test the interactions of native and glycated β2m with various highly permeable dialysis membranes. An experimental circuit with minidialyzers was used. The neutral high-flux polysulfone membrane (PS), the electronegative polymethylmetacrylate membrane (PMMA), the electronegative AN69 membrane and a modified AN69 membrane, the surface of which was neutralized with polyethyleneimine (AN69-PEI), were tested using both native β2m and the more acidic glycated β2m. Protein mass transfer and binding to the membrane were measured. Results. Mass transfer of glycated β2m was significantly decreased through all membranes tested when compared with native β2m. This result was due to the increased molecular weight of β2m, which became less permeable to porous membranes, whereas adsorption of both native and glycated β2m to membranes, due to ionic interactions, decreased similarly with AN69 and AN69-PEI, but remained unchanged with PS and PMMA. Moreover, surface neutralization of AN69 membrane did not alter its core binding capacity, since...
- Subjects
GLOBULINS; PEOPLE with diabetes; HEMODIALYSIS
- Publication
Kidney International, 2001, Vol 60, Issue 4, p1571
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.2001.00970.x