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- Title
Formation of Rabbit Reaginic Antibodies to Protein and Hapten-Protein Conjugates.
- Authors
Strannegård, Ö.; Yurchision, Alice
- Abstract
Summary. The capacities of BSA and DNP-protein conjugates to evoke reagin formation in rabbits were compared. Reagins to DNP generally appeared earlier and disappeared more rapidly from the circulation than did anti-BSA reagins. Initial formation of reagins proceeded with a logarithmic phase indicating a doubling time of 7-8 hours. Booster antigen injections resulted in some cases in a reagin response after a shorter latent phase than that observed after primary immunization. A secondary reagin response was more readily evoked in rabbits with low titres of agglutinating antibodies than in those with high titres. Anti-DNP reagins were demonstrable in a higher percentage of the injected rabbits than were anti- BSA reagins. The two types of reagins were equally sensitive to heat and 2-mercaptoethanol. A positive correlation between serum levels of anti-DNP but not anti-BSA reagins and agglutinating antibodies was demonstrated. Some evidence that a low antigen dose was more efficient than a high dose in evoking reagin formation was obtained. Treatment of rabbits with 6-mercaptopurine during the 1st week following antigen injection resulted in an increased latent phase and an enhancement of the production of anti-BSA reagins and some suppression of the formation of anti-DNP reagins.
- Subjects
IMMUNOGLOBULINS; PROTEINS; RABBITS; ANTIGENS; AGGLUTINATION; ANTIGEN-antibody reactions
- Publication
Immunology, 1969, Vol 16, Issue 3, p387
- ISSN
0019-2805
- Publication type
Article