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- Title
Acute stress to excised vocal fold epithelium from reactive oxygen species.
- Authors
Alper, Rebecca; Fu, Xue; Erickson-Levendoski, Elizabeth; Zheng, Wei; Sivasankar, Mahalakshmi
- Abstract
Objectives/Hypothesis: Vocal fold epithelium is exposed to reactive oxygen species from the inhaled environment and from tissue inflammation. The objective of this study was to explore the functional and structural consequences of reactive oxygen species exposure on vocal fold epithelium. Study Design: In vitro, prospective study design. Methods: Hydrogen peroxide (H2O2), a common reactive oxygen species, was utilized in this study. Freshly excised, viable porcine vocal fold epithelia (N = 32) were exposed to H2O2 or sham challenge for 2 hours. Electrophysiology, western blotting, and light microscopy were used to quantify the functional and structural effects of reactive oxygen species on vocal fold epithelia. Results: Exposure to reactive oxygen species did not significantly alter transepithelial resistance. There was a small, nonsignificant trend for decreased concentration of epithelial junctional complex protein with reactive oxygen species challenge. Minimal changes to the gross structural appearance of vocal fold epithelia were also noted. Conclusions: The stratified squamous epithelia of the vocal folds effectively defend against an acute reactive oxygen species challenge. The current study lays the groundwork for future investigations on the effects of reactive oxygen species on vocal fold epithelia that are compromised from phonotrauma.
- Subjects
REACTIVE oxygen species; EPITHELIUM; HYDROGEN peroxide; LARYNX injuries; ELECTROPHYSIOLOGY; VETERINARY pathophysiology; LABORATORY swine; WESTERN immunoblotting
- Publication
Laryngoscope, 2011, Vol 121, Issue 10, p2180
- ISSN
0023-852X
- Publication type
Article
- DOI
10.1002/lary.22157