We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Suppression of TGFβ-Induced Interleukin-6 Secretion by Sinulariolide from Soft Corals through Attenuation of the p38–NF-kB Pathway in Carcinoma Cells.
- Authors
Yang, Jenq-Lin; Lin, Weng-Ling; Tai, Shun-Ban; Ciou, Yi-Siang; Chung, Chih-Ling; Chen, Jih-Jung; Liu, Pei-Feng; Lin, Ming-Wei; Chen, Chun-Lin
- Abstract
Sinulariolide (SC-1) is a natural product extracted from the cultured-type soft coral Sinularia flexibilis and possesses anti-inflammation, anti-proliferative, and anti-migratory in several types of cancer cells. However, the molecular pathway behind its effects on inflammation remains poorly understood. Since inflammatory cytokines such as TGFβ, TNFα, IL-1, IL-6, and IL-8 activate transcription factors such as Smads, NF-κB, STAT3, Snail, Twist, and Zeb that drive the epithelial-to-mesenchymal transition (EMT), in this study, we focus on the investigation in effects of SC-1 on TGFβ-induced interleukin-6 (IL-6) releases in an in vitro cell culture model. We showed that both intracellular IL-6 expression and secretion were stimulated by TGFβ and associated with strong upregulation of IL-6 mRNA and increased transcription in A549 cells. SC-1 blocked TGFβ-induced secretion of IL-6 while showing no effect on the induction of fibronectin and plasminogen activator inhibitor-1 genes, indicating that SC-1 interferes with only a subset of TGFβ activities. In addition, SC-1 inhibits TGFβ-induced IL-6 by suppressing p38 MAPK signaling and subsequently inhibits NF-κB and its nuclear translocation without affecting the canonical Smad pathway and receptor turnover. Overall, these data suggest that p38 may involve in the inhibition of SC-1 in IL-6 release, thus illustrating an inhibitory effect for SC-1 in the suppression of inflammation, EMT phenotype, and tumorigenesis.
- Subjects
ALCYONACEA; INTERLEUKIN-6; SECRETION; TRANSCRIPTION factors; PLASMINOGEN activators; WNT signal transduction; FIBRONECTINS
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 14, p11656
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms241411656