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- Title
The C-terminal domain of the adenomatous polyposis coli (Apc) protein is involved in thyroid morphogenesis and function.
- Authors
Yokoyama, Atsushi; Nomura, Ryuji; Kurosumi, Masafumi; Shimomura, Atsushi; Onouchi, Takanori; Iizuka-Kogo, Akiko; Smits, Ron; Oda, Naohisa; Fodde, Riccardo; Itoh, Mitsuyasu; Senda, Takao
- Abstract
Adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of Apc, we have investigated Apc mice, which express a truncated Apc that lacks the C-terminal domain. Apc mice are tumor free and exhibit growth retardation. In the present study, we analyzed the morphology and functions of the thyroid gland in Apc mice. There was no significant difference in the basal concentration of serum thyroid hormones between Apc and Apc mice. Thyroid follicle size was significantly larger in Apc mice than in Apc mice. The extent of serum T4 elevation following exogenous thyroid-stimulating hormone (TSH) injection was lower in Apc mice than in Apc mice. TSH also induced a greater reduction in thyroid follicle size in Apc mice than in Apc mice. Analyses using immunohistochemistry and electron microscopy indicated that follicular epithelial cells in Apc mice had an enlarged rough endoplasmic reticulum of irregular shape. These results suggest that the C-terminal domain of Apc is involved in thyroid morphology and function.
- Subjects
ADENOMATOUS polyposis coli; THYROID gland; MORPHOGENESIS; TUMOR suppressor proteins; GENE expression; IMMUNOHISTOCHEMISTRY; LABORATORY mice
- Publication
Medical Molecular Morphology, 2011, Vol 44, Issue 4, p207
- ISSN
1860-1480
- Publication type
Article
- DOI
10.1007/s00795-010-0529-9