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- Title
SPR-Based Detection of ASF Virus in Cells.
- Authors
Capo, Alessandro; Calabrese, Alessia; Frant, Maciej; Walczak, Marek; Szczotka-Bochniarz, Anna; Manessis, Georgios; Bossis, Ioannis; Staiano, Maria; D'Auria, Sabato; Varriale, Antonio
- Abstract
African swine fever (ASF) is one of the most dangerous hemorrhagic infectious diseases that affect domestic and wild pigs. Currently, neither a vaccine nor effective treatments are available for this disease. As regards the degree of virulence, ASFV strains can be divided into high, moderate, or low virulence. The main detection methods are based on the use of the polymerase chain reaction (PCR). In order to prevent an uncontrolled spread of ASF, new on-site techniques that can enable the identification of an early-stage disease are needed. We have developed a specific immunological SPR-based assay for ASFV antigen detection directly in liquid samples. The developed assay allows us to detect the presence of ASFV at the dose of 103 HAD50/mL.
- Subjects
AFRICAN swine fever; IMMUNOSPECIFICITY; WILD boar; AFRICAN swine fever virus; POLYMERASE chain reaction
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 13, p7463
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23137463