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- Title
An aqueous extract of Buddleia officinalis suppresses high glucose-induced cell adhesion in human umbilical vein endothelial cells.
- Authors
Yun Jung Lee; Dae Gill Kang; Mi Kyoung Moon; Jin Sook Kim; Ho Sub Lee
- Abstract
Vascular inflammatory process has been suggested to play a key role in the initiation and progression of atherosclerosis, a major complication of diabetes mellitus. In the present study, we have investigated whether an aqueous extract of Buddleia officinalis (ABO) suppresses vascular inflammatory process induced by high glucose in the human umbilical vein endothelial cells (HUVECs). Western blot analysis revealed that incubation of HUVECs with high concentration of glucose (25 mM) increased expression levels of intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin, respectively. The expression levels of matrix metalloproteinase (MMP)-2 and 9 were also increased by treatment with high glucose in HUVECs. However, the enhanced expression levels of ICAM-1, VCAM-1, and E-selectin as well as MMP-2/-9 induced by high glucose were significantly attenuated by pretreatment with ABO in a dose-dependent manner. In addition, ABO suppressed the translocation and promoter transcriptional activity of NF-κB increased in high glucose concentration. The enhanced cell adhesion between U937 cell and HUVECs induced by high glucose was also blocked by pretreatment with ABO. Taken together, the present data suggested that ABO could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of NF-~:B activation in HUVECs.
- Subjects
ATHEROSCLEROSIS; DIABETES complications; GLUCOSE; WESTERN immunoblotting; CELL communication; METALLOPROTEINASES
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1434
- ISSN
0892-6638
- Publication type
Article