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- Title
The Small Yeast GTPase Rho5 and Its Dimeric GEF Dck1/Lmo1 Respond to Glucose Starvation.
- Authors
Schmitz, Hans-Peter; Jendretzki, Arne; Sterk, Carolin; Heinisch, Jürgen J.
- Abstract
Rho5 is a small GTPase of <italic>Saccharomyces cerevisiae</italic> and a homolog of mammalian Rac1. The latter regulates glucose metabolism and actin cytoskeleton dynamics, and its misregulation causes cancer and a variety of other diseases. In yeast, Rho5 has been implicated in different signal transduction pathways, governing cell wall integrity and the responses to high medium osmolarity and oxidative stress. It has also been proposed to affect mitophagy and apoptosis. Here, we demonstrate that Rho5 rapidly relocates from the plasma membrane to mitochondria upon glucose starvation, mediated by its dimeric GDP/GTP exchange factor (GEF) Dck1/Lmo1. A function in response to glucose availability is also suggested by synthetic genetic phenotypes of a <italic>rho5</italic> deletion with <italic>gpr1</italic>, <italic>gpa2</italic>, and <italic>sch9</italic> null mutants. On the other hand, the role of mammalian Rac1 in regulating the action cytoskeleton does not seem to be strongly conserved in <italic>S. cerevisiae</italic> Rho5. We propose that Rho5 serves as a central hub in integrating various stress conditions, including a crosstalk with the cAMP/PKA (cyclic AMP activating protein kinase A) and Sch9 branches of glucose signaling pathways.
- Subjects
GUANOSINE triphosphatase genetics; YEAST fungi genetics; GUANOSINE triphosphatase regulation; DIMERIC ions; GLUCOSE synthesis
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 8, p2186
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19082186