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- Title
Simultaneous quantitation of nicotinamide riboside and nicotinamide in dietary supplements via HPTLC–UV with confirmation by online HPTLC–ESI–MS.
- Authors
Neamţu, Andreea Silvia; Biţă, Andrei; Scorei, Ion Romulus; Rău, Gabriela; Bejenaru, Ludovic Everard; Bejenaru, Cornelia; Rogoveanu, Otilia-Constantina; Oancea, Carmen Nicoleta; Radu, Antonia; Pisoschi, Cătălina Gabriela; Neamţu, Johny; Mogoşanu, George Dan
- Abstract
The identification and quantitation of nicotinamide riboside (NAR) and its main related compound (nicotinamide) were achieved using high-performance thin-layer chromatography (HPTLC)–ultraviolet (UV) densitometry with confirmation by online electrospray ionization (ESI)–mass spectrometry (MS). As the stationary phase, HPTLC Si 60 F254 glass plates were employed; the mobile phase was ethanol–1 M ammonium acetate–formic acid (7:1:0.1, v/v/v). No derivatization was applied, and UV densitometry was performed in the absorbance mode (270 nm). The method was validated by specificity, linearity, accuracy, precision, and robustness.
- Subjects
NICOTINAMIDE; DIETARY supplements; DENSITOMETRY
- Publication
Acta Chromatographica, 2020, Vol 32, Issue 2, p128
- ISSN
1233-2356
- Publication type
Article
- DOI
10.1556/1326.2019.00600