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- Title
Capping of actin filaments by vinculin activated by the Shigella IpaA carboxyl-terminal domain
- Authors
Ramarao, Nalini; Le Clainche, Christophe; Izard, Tina; Bourdet-Sicard, Raphaëlle; Ageron, Elisabeth; Sansonetti, Philippe J.; Carlier, Marie-France; Tran Van Nhieu, Guy
- Abstract
Abstract: Shigella, the causative agent of bacillary dysentery, invades epithelial cells. Upon bacterial–cell contact, the type III bacterial effector IpaA binds to the cytoskeletal protein vinculin to promote actin reorganization required for efficient bacterial uptake. We show that the last 74 C-terminal residues of IpaA (A559) bind to human vinculin (HV) and promotes its association with actin filaments. Polymerisation experiments demonstrated that A559 was sufficient to induce HV-dependent partial capping of the barbed ends of actin filaments. These results suggest that IpaA regulates actin polymerisation/depolymerisation at sites of Shigella invasion by modulating the barbed end capping activity of vinculin.
- Subjects
EPITHELIAL cells; CYTOSKELETAL proteins; POLYMERIZATION; SHIGELLA
- Publication
FEBS Letters, 2007, Vol 581, Issue 5, p853
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.01.057