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- Title
Identification of hepatic peroxisomal phospholipase A<sub>2</sub> and characterization of arachidonic acid-containing choline glycerophospholipids in hepatic peroxisomes
- Authors
Yang, Jingyue; Han, Xianlin; Gross, Richard W.
- Abstract
Recently, a sequence encoding a novel mammalian calcium-independent phospholipase A2 (iPLA2γ) was identified in the human genome and subsequently cloned and expressed in Sf9 insect cells. Unexpectedly, expression studies in recombinant systems demonstrated the usage of multiple translation initiation codons resulting in different polypeptides. Herein, we demonstrate that hepatic iPLA2γ is localized to rat liver peroxisomes, possesses a molecular mass of 63 kDa and that peroxisomal membranes are highly enriched in arachidonic acid-containing phospholipids. Collectively, these results provide the first demonstration of iPLA2γ in mammalian tissue and suggest the possibility that iPLA2γ can contribute to lipid second messenger generation by hydrolysis of peroxisomal arachidonic acid-containing phospholipids.
- Subjects
PHOSPHOLIPASE A2; PROTEINS
- Publication
FEBS Letters, 2003, Vol 546, Issue 2/3, p247
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00581-7