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- Title
Investigation of the biosynthesis of acetyl-CoA and oxaloacetic acid from pyruvic acid and the quantitative evaluation of incorporated C-labeled l-alanine in Arthrobacter hyalinus.
- Authors
Iida, Katsumi
- Abstract
Studies on the contribution to acetyl-CoA and oxaloacetic acid from the pyruvic acid transformation from l-alanine in Arthrobacter hyalinus were conducted by means of feeding experiments with l-[1-C]alanine and l-[3-C]alanine, followed by an analysis of the labeling patterns of coproporphyrinogen III using C NMR spectroscopy. The results demonstrated that l-alanine was transformed via pyruvic acid to both acetyl-CoA and oxaloacetic acid. Additionally, the quantitative analysis indicated that pyruvic acid was transformed to acetyl-CoA and oxaloacetic acid in the ratio of 1:0.8.
- Subjects
ACETYLCOENZYME A; OXALACETATE; PYRUVIC acid; ARTHROBACTER; NUCLEAR magnetic resonance spectroscopy; QUANTITATIVE research; ALANINE
- Publication
Journal of Radioanalytical & Nuclear Chemistry, 2014, Vol 301, Issue 3, p897
- ISSN
0236-5731
- Publication type
Article
- DOI
10.1007/s10967-014-3172-4