We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Engineering Electron Transfer Pathway of Cytochrome P450s.
- Authors
He, Jingting; Liu, Xin; Li, Chun
- Abstract
Cytochrome P450s (P450s), a superfamily of heme-containing enzymes, existed in animals, plants, and microorganisms. P450s can catalyze various regional and stereoselective oxidation reactions, which are widely used in natural product biosynthesis, drug metabolism, and biotechnology. In a typical catalytic cycle, P450s use redox proteins or domains to mediate electron transfer from NAD(P)H to heme iron. Therefore, the main factors determining the catalytic efficiency of P450s include not only the P450s themselves but also their redox-partners and electron transfer pathways. In this review, the electron transfer pathway engineering strategies of the P450s catalytic system are reviewed from four aspects: cofactor regeneration, selection of redox-partners, P450s and redox-partner engineering, and electrochemically or photochemically driven electron transfer.
- Subjects
CHARGE exchange; STEREOSELECTIVE reactions; DRUG metabolism; PROTEIN domains; ENGINEERING; NAD (Coenzyme)
- Publication
Molecules, 2024, Vol 29, Issue 11, p2480
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules29112480