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- Title
NMR-Based Analysis of Plasma Lipoprotein Subclass and Lipid Composition Demonstrate the Different Dietary Effects in ApoE-Deficient Mice.
- Authors
Yang, Cheng-Hung; Ho, Yu-Hsuan; Tang, Hsiang-Yu; Lo, Chi-Jen
- Abstract
Plasma lipid levels are commonly measured using traditional methods such as triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), and cholesterol (CH). However, the use of newer technologies, such as nuclear magnetic resonance (NMR) with post-analysis platforms, has made it easier to assess lipoprotein profiles in research. In this study involving ApoE-deficient mice that were fed high-fat diets, significant changes were observed in TG, CH, free cholesterol (FC), and phospholipid (PL) levels within the LDL fraction. The varied proportions of TG in wild-type mice and CH, FC, and PL in ApoE-/- mice were strikingly different in very low-density lipoproteins (VLDL), LDL, intermediate-density lipoprotein (IDL), and HDL. This comprehensive analysis expands our understanding of lipoprotein subfractions and the impacts of the APOE protein and high-fat diet in mouse models. The new testing method allows for a complete assessment of plasma lipids and their correlation with genetic background and diet in mice.
- Subjects
BLOOD lipids; LOW density lipoproteins; NUCLEAR magnetic resonance; HIGH-fat diet; HIGH density lipoproteins; MICE; BLOOD lipoproteins
- Publication
Molecules, 2024, Vol 29, Issue 5, p988
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules29050988