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- Title
Bacteriocin Activity of Enterococci from Rabbits.
- Authors
M. Simonová; A. Lauková
- Abstract
Abstract??The antimicrobial spectrum of bacteriocin-producing enterococcal isolates from rabbits faeces, the biochemical characterization of bacteriocins, and their molecular mass and the presence of structural genes for bacteriocin production were investigated. Among enterococci selected from rabbit faeces, six strains ofEnterococcus faecium(EF2019, EF1819, EF2119, EF1839, EF529, EF24/10) showed inhibitory activity against the indicatorsE. aviumEA5,Listeria innocuaLMG13568 andListeriamonocytogenesCCM4699 and against other enterococci and staphylococci tested. The molecular mass of bacteriocin-like substances ranged from 3 to 10 kDa. The presence of the structural genes for enterocins (ent) A, P and L50B was detected in all enterococci tested. However, no strain possessed the gene for ent B.E.faeciumEF2019 showed the broadest inhibitory activity. A proteinaceous substance produced by the EF2019 strain was partially purified. This is a thermostable substance that is stable at pH 4.0, 7.0 and 9.0. Its production starts in the early logarithmic growth phase and culminates in the late logarithmic growth phase of the EF2019 strain. Partially purified bacteriocin (PPB) EF2019 added to the growing strainL. innocuaLMG13568 (after 4 h) inhibited its growth as early as 1 h after addition with a decrease of 1.5 log cycles (5 h of cultivation). This effect was extended up to 24 h. The bacteriocinogenicE. faeciumEF2019 strain and/or bacteriocin EF2019 could be utilized against contaminant bacteria, e.g. in rabbitries. More detailed studies underin vitroandin vivoconditions are in progress.
- Subjects
STREPTOCOCCACEAE; EUROPEAN rabbit; ENTEROCOCCUS; ANTIBACTERIAL agents
- Publication
Veterinary Research Communications, 2007, Vol 31, Issue 2, p143
- ISSN
0165-7380
- Publication type
Article