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- Title
Can laser therapy modify the secretion of the tissue-type plasminogen activator and its inhibitor in an endothelial cell culture under hyperglycemia?
- Authors
Góralczyk, Krzysztof; Szymańska, Justyna; Ziętek, Zbigniew; Szot, Katarzyna; Fisz, Jacek
- Abstract
Introduction: The effect of low-level laser therapy on the secretion of tissue-type plasminogen activator (t-PA) and its inhibitor (PAI-1) in an endothelial cell (EC) culture under hyperglycemia is the subject of the presented work. Hyperglycemia associated with diabetes causes vascular EC dysfunction. Low-level laser therapy is a good method to support the pharmacological treatment of diabetes complications. Materials and methods: We used lasers of 2 wavelengths: 635 nm and 830 nm with dose of 2 J/cm2. The experiment was performed in vitro in 4 groups of EC: 1 - no glucose in culture medium, no irradiation (control), 2 - glucose, no irradiation, 3 - glucose, laser 635 nm, 4 - glucose, laser 830 nm. After 2 irradiations, cells were counted and the t-PA and PAI-1 antigen (Ag) concentration in the supernatant was measured. Results: In group 2, we observed a statistically significant lower number of cells (p < 0.0001) and a higher concentration of t-PA:Ag and PAI-1:Ag (p < 0.05) compared to the control group. However, in groups 3 and 4, the number of cells increased and the concentration of t-PA:Ag and PAI-1:Ag decreased compared to group 2 and nearly reached the values in the control group. Conclusions: Hyperglycemia affects the fibrinolytic activity of ECs which is manifested by a significant increase in t-PA:Ag and PAI:Ag concentrations - recognized markers of endothelial damage. Irradiation of ECs by a low-power laser caused attenuation of the adverse effects of hyperglycemia. A tendency towards a decrease in t-PA:Ag and PAI-1:Ag concentration in the supernatant was observed with a significant increase in the number of cells to values close to control.
- Subjects
LASER therapy; TISSUE plasminogen activator; CIRCULATING anticoagulants; HYPERGLYCEMIA treatment; GLUCOSE metabolism disorders
- Publication
Pomeranian Journal of Life Sciences, 2021, Vol 67, Issue 2, p1
- ISSN
2450-4637
- Publication type
Article
- DOI
10.21164/pomjlifesci.728