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- Title
The 24p3 Gene Is Induced during Involution of the Mammary Gland and Induces Apoptosis of Mammary Epithelial Cells.
- Authors
Jin-Jong Bong; Myung-Bok Seol; Hyung-Ha Kim; Oksoo Han; Kyoungwhan Back; Myunggi Baik
- Abstract
To understand the molecular mechanism of mammary gland involution we identified involution-induced clones by differential screening of a mouse mammary gland cDNA library. Characterization of clones by sequencing and Northern analysis showed that expression of 24p3 was induced during involution of the mammary gland. RNA in situ hybridization showed that it was mainly expressed in the secretory epithelial cells surrounding the lumen of the mammary gland alveoli. Induction of 24p3 was also observed in apoptotic HC11 mammary epithelial cells under serum starvation. In these cells, dexamethasone Increased 24p3 gene expression four-fold. Transient expression of 24p3 increased the percentage of apoptotic cells 3- to 4-fold over a period of 3 days after transfection. This study provides evidence that overexpression of 24p3 gene can induce apoptosis of mammary epithelial cells.
- Subjects
MAMMARY glands; RNA; IN situ hybridization; EPITHELIAL cells; GENE expression
- Publication
Molecules & Cells (Springer Nature), 2004, Vol 17, Issue 1, p29
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13001-9