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- Title
Association of Circulating Transfer RNA fragments with antibody response to <italic>Mycoplasma bovis</italic> in beef cattle.
- Authors
Casas, Eduardo; Cai, Guohong; Kuehn, Larry A.; Register, Karen B.; McDaneld, Tara G.; Neill, John D.
- Abstract
Background: High throughput sequencing allows identification of small non-coding RNAs. Transfer RNA Fragments are a class of small non-coding RNAs, and have been identified as being involved in inhibition of gene expression. Given their role, it is possible they may be involved in mediating the infection-induced defense response in the host. Therefore, the objective of this study was to identify 5′ transfer RNA fragments (tRF5s) associated with a serum antibody response to <italic>M. bovis</italic> in beef cattle. Results: The tRF5s encoding alanine, glutamic acid, glycine, lysine, proline, selenocysteine, threonine, and valine were associated (<italic>P</italic> < 0.05) with antibody response against <italic>M. bovis.</italic> tRF5s encoding alanine, glutamine, glutamic acid, glycine, histidine, lysine, proline, selenocysteine, threonine, and valine were associated (<italic>P</italic> < 0.05) with season, which could be attributed to calf growth. There were interactions (<italic>P</italic> < 0.05) between antibody response to <italic>M. bovis</italic> and season for tRF5 encoding selenocysteine (anticodon UGA), proline (anticodon CGG), and glutamine (anticodon TTG). Selenocysteine is a rarely used amino acid that is incorporated into proteins by the opal stop codon (UGA), and its function is not well understood. Conclusions: Differential expression of tRF5s was identified between ELISA-positive and negative animals. Production of tRF5s may be associated with a host defense mechanism triggered by bacterial infection, or it may provide some advantage to a pathogen during infection of a host. Further studies are needed to establish if tRF5s could be used as a diagnostic marker of chronic exposure.
- Subjects
TRANSFER RNA; BEEF cattle diseases; ANTIBODY formation; MYCOPLASMA bovis; GENE expression in mammals
- Publication
BMC Veterinary Research, 2018, Vol 14, p1
- ISSN
1746-6148
- Publication type
Article
- DOI
10.1186/s12917-018-1418-z