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- Title
Molecular Cloning and Expression of Major Histocompatibility Complex Class Ia Gene of Swamp Eel (Monopterus albus).
- Authors
Ting-shuang Pan; He Jiang; Guo-qing Duan; Yu-ting Hu; Jun Ling; Hua-xing Zhou
- Abstract
Major histocompatibility complex (MHC) plays an important role in the immune response of vertebrates. In this study, we isolated MHC class Ia gene from swamp eel (Monopterus albus) by rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). In order to study the function of MHC Ia in swamp eel, tissue distribution and immune response of the MHC Ia after bacterial challenge were analyzed. All the characteristic features of MHC Ia chain structure were identified by the deduced amino acid sequence, including the leader peptide, α1, α2, and α3 domains, connecting peptide, transmembrane and cytoplasmic regions, conserved cysteines, and Nglycosylation site. The deduced amino acid sequence of the MHC Ia shares 27.3% to 69.6% similarity with those of other vertebrates. Quantitative realtime PCR (qRT-PCR) demonstrated that MHC Iα gene was ubiquitously expressed in ten tissues, comparatively higher in immune related tissues, including the liver and intestine. Challenge of M. albus with the extracellular pathogen, Aeromonas hydrophila, resulted in a significant increase in the expression of MHC Ia within 72 h after infection and these high levels were maintained for 72 h in kidneys, spleen, and skin, whereas in the intestine and liver, it was found first in the form of up-regulated and then down-regulated gene. These results demonstrated that MHC Ia gene played an important role in response to bacterial infection in M. albus.
- Subjects
MAJOR histocompatibility complex; MONOPTERUS albus; IMMUNE response; AEROMONAS hydrophila; BACTERIAL diseases
- Publication
Israeli Journal of Aquaculture-Bamidgeh, 2018, Vol 70, p1
- ISSN
0792-156X
- Publication type
Article