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- Title
Single nucleotide polymorphism (SNP) discovery in porcine expressed genes.
- Authors
Fahrenkrug, S. C; Freking, B. A; Smith, T. P. L; Rohrer, G. A; Keele, J. W
- Abstract
High-throughput genotyping of swine populations is a potentially efficient method for establishing animal lineage and identification of loci important to animal health and efficient pork production. Markers were developed based upon single nucleotide polymorphisms (SNPs), which are abundant and amenable to automated genotyping platforms. The focus of this research was SNP discovery in expressed porcine genes providing markers to develop the porcine/human comparative map. Locus specific amplification (LSA) and comparative sequencing were used to generate PCR products and allelic information from parents of a swine reference family. Discovery of 1650 SNPs in 403 amplicons and strategies for optimizing LSA-based SNP discovery using alternative methods of PCR primer design, data analysis, and germplasm selection that are applicable to other populations and species are described. These data were the first large-scale assessment of frequency and distribution of porcine SNPs.
- Subjects
NUCLEOTIDE sequence; GENETIC polymorphisms; GENE expression; SWINE; GENETICS
- Publication
Animal Genetics, 2002, Vol 33, Issue 3, p186
- ISSN
0268-9146
- Publication type
Article
- DOI
10.1046/j.1365-2052.2002.00846.x