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- Title
IL-4 down-regulates the surface expression of CD5 on B cells and inhibits spontaneous immunoglobulin and IgM-rheumatoid factor production in patients with rheumatoid arthritis.
- Authors
Hidaka, T.; Kitani, A.; Hara, M.; Harigai, M.; Suzuki, K.; Kawaguchi, Y.; Ishizuka, T.; Kawagoe, M.; Nakamura, H.
- Abstract
There is evidence to suggest that CD5+ B cells may be associated with autoimmunity, e.g. they are increased in patients with rheumatoid arthritis (RA). In this study, we found that the expression of CD5 on RA B cells increased spontaneously, following culture for up to 4 days <em>in vitro</em> in the absence of T cells, supporting the idea that the CD5+ B cell possesses distinctive features. The spontaneous increase of CD5 expression was down-regulated by recombinant IL-4 (rIL-4). Other cytokines studied (rIL-1α, rIL-2, rIL-5, rIL-6) did not alter CD5 expression. Studies of antibody production showed that rIL-4 could reduce spontaneous production of total IgG and IgM in non-stimulated RA T plus B cell cultures. Spontaneous production of IgM rheumatoid factor (IgM-RF), measured by a newly developed avidin-biotin complex ELISA, was also reduced by rIL-4. Furthermore, rIL-4 reduced the increase in IgM-RF production observed on stimulation with <em>Staphylococcus aureus</em> Cowan I (SAC) or pokeweed mitogen (PWM). Thus, IL-4 might act as a regulator of the development of abnormal B cell differentiation in patients with RA.
- Subjects
RHEUMATOID arthritis; B cells; INTERLEUKIN-4; ANTIGEN presenting cells; RHEUMATOID factor; IMMUNOGLOBULIN M; IMMUNOGLOBULIN G; STAPHYLOCOCCUS aureus
- Publication
Clinical & Experimental Immunology, 1992, Vol 89, Issue 2, p223
- ISSN
0009-9104
- Publication type
Article