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- Title
Frequent pUL27 Variations in HIV-Infected Patients.
- Authors
Mirarab, azam; Mohebbi, alireza; Moradi, abdolvahab; Javid, Naeme; Vakili, Mohammad ali; Tabarraei, alijan
- Abstract
Objective: Drug-resistant isolates of human cytomegalovirus (HCMV) have led to the development of new anti-HCMV drugs. Maribavir (MBV) is a novel inhibitor of the HCMV viral kinase. Resistance to MBV is mapped to gene UL27, a viral nuclear protein. In this study, we investigated UL27 polymorphisms in MBV-naive HIV-positive and HCMV congenitally infected clinical samples. Methods: DNA was extracted from 20 CMV-positive HIV (9/20) and congenitally infected (11/20) patients and used for UL27 polymerase chain reaction amplification. Sanger sequencing and multiple sequence alignment of products was performed. Results: K90 was the most prevalent polymorphism in both HIV-positive and congenitally infected patients. Polymorphisms Q54, D123, and R107 (10%) were seen in more than one sample. There were significantly more polymorphisms in the HIVpositive samples (p = 0.038). Conclusion: HCMV pUL27 is highly variable in adult immunocompromised HIV-positive patients.
- Subjects
HUMAN cytomegalovirus; DRUG resistance in microorganisms; HIV-positive persons; GENETIC polymorphisms; POLYMERASE chain reaction; VIRAL proteins; KINASES; HEALTH
- Publication
Intervirology, 2017, Vol 59, Issue 5/6, p262
- ISSN
0300-5526
- Publication type
Article
- DOI
10.1159/000471484