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- Title
Desenvolvimento e validação de método para separação de isoflavonas em extrato seco de soja.
- Authors
Ribani, Marcelo; Collins, Carol H.; Bottoli, Carla Beatriz Grespan
- Abstract
Isoflavones are phenolic compounds naturally found in some plants and are known as phytoestrogens, compounds found in plant-derived foods, primarily in products based on soybeans. In this work an analytical methodology for the High Performance Liquid Chromatographic separation and quantification of isoflavones present in dry soy extract was developed. In order to validate the method, parameters such as selectivity, precision, accuracy, linearity and working range, limit of detection (LOD), limit of quantification (LOQ) and robustness were evaluated. Acceptability criteria for each parameter were established. The methodology proved to be suitable for determination and quantification of the glycoside and aglycone isoflavones found in dry soy extract.
- Publication
Revista Ciência e Natura, 2014, Vol 36, Issue 3, p501
- ISSN
0100-8307
- Publication type
Article
- DOI
10.5902/2179460X12817