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- Title
Evaluation of a Novel Synthetic Peptide Derived from Cytolytic Mycotoxin Candidalysin.
- Authors
Cardoso, Pedro Henrique de Oliveira; Boleti, Ana Paula de Araújo; Silva, Patrícia Souza e; Mukoyama, Lincoln Takashi Hota; Guindo, Alexya Sandim; Moraes, Luiz Filipe Ramalho Nunes de; Oliveira, Caio Fernando Ramalho de; Macedo, Maria Ligia Rodrigues; Carvalho, Cristiano Marcelo Espínola; de Castro, Alinne Pereira; Migliolo, Ludovico
- Abstract
The importance of neuroinflammation in neurology is becoming increasingly apparent. In addition to neuroinflammatory diseases such as multiple sclerosis, the role of neuroinflammation has been identified in many non-inflammatory neurological disorders such as stroke, epilepsy, and cancer. The immune response within the brain involves the presence of CNS resident cells; mainly glial cells, such as microglia, the CNS resident macrophages. We evaluated the peptide Ca-MAP1 bioinspired on the C. albicans immature cytolytic toxin candidalysin to develop a less hemolytic peptide with anti-neuroinflammatory, antibacterial, and cytotoxic activity against tumor cells. In silico and in vitro studies were performed at various concentrations. Ca-MAP1 exhibits low hemolytic activity at lower concentrations and was not cytotoxic to MRC-5 and BV-2 cells. Ca-MAP1 showed activity against Acinetobacter baumannii, Escherichia coli ATCC, E. coli KPC, Klebsiella pneumoniae ATCC, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC. Furthermore, Ca-MAP1 exhibits anti-neuroinflammatory activity in the BV-2 microglia model, with 93.78% inhibition of nitrate production at 18.1 µM. Ca-MAP1 presents cytotoxic activity against tumor cell line NCI-H292 at 36.3 μM, with an IC50 of 38.4 µM. Ca-MAP1 demonstrates results that qualify it to be evaluated in the next steps to promote the control of infections and provide an alternative antitumor therapy.
- Subjects
PEPTIDES; ESCHERICHIA coli; NEUROGLIA; KLEBSIELLA pneumoniae; PSEUDOMONAS aeruginosa; NEUROCYSTICERCOSIS; TOXINS; ACINETOBACTER baumannii
- Publication
Toxins, 2022, Vol 14, Issue 10, pN.PAG
- ISSN
2072-6651
- Publication type
Article
- DOI
10.3390/toxins14100696