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- Title
Improvement of Oil Degradation and MEL Production in a Yeast Strain, Pseudozyma tsukubaensis, by Translation Elongation Factor 1 Promoter-driven Expression of a Lipase.
- Authors
Azusa Saika; Hideaki Koike; Shuhei Yamamoto; Tomohiro Sugahara; Akio Kawahara; Atsushi Sogabe; Tomotake Morita
- Abstract
The basidiomycetous yeast Pseudozyma tsukubaensis produces a mannosylerythritol lipid (MEL) homologue, a diastereomer type of MEL-B, from olive oil. In a previous study, MEL-B production was increased by the overexpression of lipase PaLIPAp in P. tsukubaensis 1E5, through the enhancement of oil consumption. In the present study, RNA sequence analysis was used to identify a promoter able to induce high-level PaLIPA expression. The recombinant strain, expressing PaLIPA via the translation elongation factor 1 alpha/Tu promoter, showed higher lipase activity, rates of oil degradation, and MEL-B production than the strain which generated in our previous study.
- Subjects
RNA sequencing; OLIVE oil; YEAST; PETROLEUM; OIL consumption; LIPASES
- Publication
Journal of Oleo Science, 2022, Vol 71, Issue 9, p1421
- ISSN
1345-8957
- Publication type
Article
- DOI
10.5650/jos.ess22089