We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MHC haplotype frequencies in a UK breeding colony of Mauritian cynomolgus macaques mirror those found in a distinct population from the same geographic origin.
- Authors
Mee, Edward T.; Badhan, Anjna; Karl, Julie A.; Wiseman, Roger W.; Cutler, Keith; Knapp, Leslie A.; Almond, Neil; O'Connor, David H.; Rose, Nicola J.
- Abstract
Background Mauritian cynomolgus macaques have greatly restricted genetic diversity in the MHC region compared to other non-human primates; however, the frequency of common MHC haplotypes among captive-bred populations has not been reported. Methods Microsatellite PCR was used to determine MHC haplotype frequencies among captive macaques at a UK breeding facility. Allele-specific PCR and reference strand conformational analysis were used to determine the allele expression profile of a subset of animals. Results Haplotypes H3 (21%) and H1 (19%) were most common in the captive population of Mauritian cynomolgus macaques. Predicted alleles were detected by allele-specific PCR-SSP in 98% of animals. Allele expression profiles were similar in animals with identical haplotypes. Conclusions Mauritian cynomolgus macaques in the UK breeding facility have restricted MHC diversity comparable to a previously described population. Microsatellite-derived haplotypes are highly predictive of allele expression. A selective breeding program has been established to produce MHC-identical animals for biomedical research.
- Subjects
KRA; MICROSATELLITE repeats; MACAQUES; CONFORMATIONAL analysis; BREEDING; MEDICAL research; CERCOPITHECIDAE
- Publication
Journal of Medical Primatology, 2009, Vol 38, Issue 1, p1
- ISSN
0047-2565
- Publication type
Article
- DOI
10.1111/j.1600-0684.2008.00299.x