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- Title
Nasosorption as a Minimally Invasive Sampling Procedure: Mucosal Viral Load and Inflammation in Primary RSV Bronchiolitis.
- Authors
Thwaites, Ryan S.; Ito, Kazuhiro; Chingono, Jasmine M. S.; Coates, Matthew; Jarvis, Hannah C.; Tunstall, Tanushree; Anderson-Dring, Lauren; Cass, Lindsey; Rapeport, Garth; Openshaw, Peter J.; Nadel, Simon; Hansel, Trevor T.
- Abstract
<bold>Background: </bold>Existing respiratory mucosal sampling methods are flawed, particularly in a pediatric bronchiolitis setting.<bold>Methods: </bold>Twenty-four infants with bronchiolitis were recruited: 12 were respiratory syncytial virus (RSV)-positive, 12 were RSV-negative. Infants were sampled by nasosorption and nasopharyngeal aspiration (NPA).<bold>Results: </bold>Nasosorption was well tolerated and identified all RSV+ samples. RSV load measured by nasosorption (but not NPA) correlated with length of hospital stay (P = .04) and requirement for mechanical ventilation (P = .03). Nasosorption (but not NPA) levels of interferon γ, interleukin 1β, CCL5/RANTES, and interleukin 10 (IL-10) were elevated in RSV+ bronchiolitis (all P < .05), furthermore CCL5 and IL-10 correlated with RSV load (P < .05).<bold>Conclusions: </bold>Nasosorption allowed measurement of RSV load and the mucosal inflammatory response in infants.
- Subjects
ENGLAND; LAPAROSCOPIC surgery; BRONCHIOLITIS; INFANT diseases; INTERFERONS; INTERLEUKIN-10; ARTIFICIAL respiration; BRONCHIOLE diseases; CYTOKINES; INFLAMMATION; INTERLEUKINS; NASAL mucosa; RESPIRATORY syncytial virus; VIRAL load; CASE-control method; RESPIRATORY syncytial virus infections; DIAGNOSIS
- Publication
Journal of Infectious Diseases, 2017, Vol 215, Issue 8, p1240
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jix150