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- Title
Two Novel Alliin Lyase (Alliinase) Genes from Twisted-Leaf Garlic (Allium obliquum) and Mountain Garlic (Allium senescens ssp. montanum).
- Authors
Drugă, Bogdan; Şuteu, Dana; Roşca-Casian, Oana; Pârvu, Marcel; Dragoş, Nicolae
- Abstract
Alliinase (Alliin lyase EC 4.4.1.4), a pyridoxal phosphate-dependent lyase, represents one of the major protein components of Allium species. The enzyme is a homodimeric glycoprotein and catalyzes the synthesis of allicin (diallyl thiosulinate, a biologically active compound), pyruvate, and ammonia starting from the specific non-protein sulfur-containing amino acid alliin ((+S)-allyl-L-cysteine sulfoxide). Using newly developed specific primers two new alliinase genes from Allium obliquum and Allium senescens ssp. montanum were amplified and sequenced, as well as their homologs, from Allium fistulosum and Allium schoenoprasum. The G+C content of the alliinase region ranges between that of other dicot plants and that reported in monocot cereal plants, in all four species. Investigations of gene expression revealed a significantly higher enzyme expression level in bulbs than in leaves in all four taxa. The deduced alliinase sequences displayed a high variability among different species, since the lowest sequence similarity was found to be 55.5% between Allium senescens ssp. montanum and Allium cepa, while the highest similarity is 77.5%, between Allium senescens ssp. montanum and Allium istulosum. Leucine is the most common amino acid in all four alliinases, while cysteine is also more frequent than in other enzymes, suggesting a high stability of the molecules due to the possible disulfide bonds.
- Subjects
ALLIIN lyase; GENES; GARLIC; PHOSPHATES; ALLIUM; ENZYMES; GLYCOPROTEINS; AMMONIA
- Publication
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2011, Vol 39, Issue 2, p293
- ISSN
0255-965X
- Publication type
Article
- DOI
10.15835/nbha3926355