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- Title
The PGM3 gene encodes the major phosphoribomutase in the yeast Saccharomyces cerevisiae
- Authors
Walther, Thomas; Baylac, Audrey; Alkim, Ceren; Vax, Amélie; Cordier, Hélène; François, Jean Marie
- Abstract
Abstract: The phosphoglucomutases (PGM) Pgm1, Pgm2, and Pgm3 of the yeast Saccharomyces cerevisiae were tested for their ability to interconvert ribose-1-phosphate and ribose-5-phosphate. The purified proteins were studied in vitro with regard to their kinetic properties on glucose-1-phosphate and ribose-1-phosphate. All tested enzymes were active on both substrates with Pgm1 exhibiting only residual activity on ribose-1-phosphate. The Pgm2 and Pgm3 proteins had almost equal kinetic properties on ribose-1-phosphate, but Pgm2 had a 2000 times higher preference for glucose-1-phosphate when compared to Pgm3. The in vivo function of the PGMs was characterized by monitoring ribose-1-phosphate kinetics following a perturbation of the purine nucleotide balance. Only mutants with a deletion of PGM3 hyper-accumulated ribose-1-phosphate. We conclude that Pgm3 functions as the major phosphoribomutase in vivo.
- Subjects
PHOSPHOGLUCOMUTASE genetics; GENE expression; SACCHAROMYCES cerevisiae; RIBOSE phosphates; PURINE nucleotides; ENZYME activation
- Publication
FEBS Letters, 2012, Vol 586, Issue 23, p4114
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.09.042