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- Title
EPR characterization of the new Qrc complex from sulfate reducing bacteria and its ability to form a supercomplex with hydrogenase and TpIc <sub>3</sub>
- Authors
Venceslau, Sofia S.; Matos, Daniela; Pereira, Inês A.C.
- Abstract
Abstract: The Quinone-reductase complex (Qrc) is a respiratory complex with Type I cytochrome c 3:menaquinone reductase activity, recently described in sulfate-reducing bacteria. Qrc is related to the complex iron–sulfur molybdoenzyme family and to the alternative complex III. In this work we report a detailed characterization of the redox properties of the metal cofactors of Qrc using EPR spectroscopy, which allowed the determination of the reduction potentials of five out of six hemes c, one [3Fe–4S]1+/0 center and the three [4Fe–4S]2+/1+ centers. In addition, we show that Qrc forms a supercomplex with [NiFe] hydrogenase and TpIc 3, its physiological electron donors. Structured summary of protein interactions: NiFe Hydrogenase physically interacts with Qrc by native page (View interaction).
- Subjects
QUINONE; CYTOCHROME c; VITAMIN K2; HYDROGENASE; DESULFOVIBRIO; COMPLEX compounds; ELECTRON paramagnetic resonance spectroscopy; PROTEIN-protein interactions
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2177
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.054