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- Title
Small mitochondrial ARF (smARF) is located in both the nucleus and cytoplasm, induces cell death, and activates p53 in mouse fibroblasts
- Authors
Ueda, Yuko; Koya, Terutsugu; Yoneda-Kato, Noriko; Kato, Jun-ya
- Abstract
Abstract: The ARF transcript produces two proteins, the full-length ARF, p19ARF, and a short mitochondrial version, smARF. To explore the functional difference between the two, we generated GFP-fused expression vectors for each protein and introduced them into NIH3T3 murine fibroblasts, which sustains a global deletion in the INK4a locus but contains a functional p53 gene. GFP-p19ARF was located within the nucleolus as previously reported, whereas GFP-smARF was detected mainly in the nucleoplasm. GFP-smARF induced cell death although to a slightly lesser extent than p19ARF. GFP-smARF stabilized p53 thereby inducing expression of the target genes, MDM2 and p21. We suggest that smARF has functions other than mitochondria-mediated autophagy, and induces p53 expression and cell death via a novel mechanism.
- Subjects
FIBROBLASTS; P53 protein; CELL death; CYTOPLASM
- Publication
FEBS Letters, 2008, Vol 582, Issue 10, p1459
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.03.032