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- Title
Site specific phosphorylation of cytochrome c oxidase subunits I, IVi1 and Vb in rabbit hearts subjected to ischemia/reperfusion
- Authors
Fang, Ji-Kang; Prabu, Subbuswamy K.; Sepuri, Naresh B.; Raza, Haider; Anandatheerthavarada, Hindupur K.; Galati, Domenico; Spear, Joseph; Avadhani, Narayan G.
- Abstract
Abstract: We have mapped the sites of ischemia/reperfusion-induced phosphorylation of cytochrome c oxidase (CcO) subunits in rabbit hearts by using a combination of Blue Native gel/Tricine gel electrophoresis and nano-LC–MS/MS approaches. We used precursor ion scanning combined with neutral loss scanning and found that mature CcO subunit I was phosphorylated at tandem Ser115/Ser116 positions, subunit IVi1 at Thr52 and subunit Vb at Ser40. These sites are highly conserved in mammalian species. Molecular modeling suggests that phosphorylation sites of subunit I face the inter membrane space while those of subunits IVi1 and Vb face the matrix side.
- Subjects
PROTEIN kinases; CHEMICAL reactions; ELECTROPHORESIS; ISCHEMIA
- Publication
FEBS Letters, 2007, Vol 581, Issue 7, p1302
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.02.042