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- Title
MRI detection of transcriptional regulation of gene expression in transgenic mice.
- Authors
Cohen, Batya; Ziv, Keren; Plaks, Vicki; Israely, Tomer; Kalchenko, Vyacheslav; Harmelin, Alon; Benjamin, Laura E; Neeman, Michal
- Abstract
Ferritin, the iron storage protein, was recently suggested to be a candidate reporter for the detection of gene expression by magnetic resonance imaging (MRI). Here we report the generation of TET:EGFP-HAferritin (tet-hfer) transgenic mice, in which tissue-specific inducible transcriptional regulation of expression of the heavy chain of ferritin could be detected in vivo by MRI. We show organ specificity by mating the tet-hfer mice with transgenic mice expressing tetracycline transactivator (tTA) in liver hepatocytes and in vascular endothelial cells. Tetracycline-regulated overexpression of ferritin resulted in specific alterations of the transverse relaxation rate (R2) of water. Transgene-dependent changes in R2 were detectable by MRI in adult mice, and we also found fetal developmental induction of transgene expression in utero. Thus, the tet-hfer MRI reporter mice provide a new transgenic mouse platform for in vivo molecular imaging of reporter gene expression by MRI during both embryonic and adult life.
- Subjects
MAGNETIC resonance imaging; TRANSGENIC mice; TRANSCRIPTION factors; FERRITIN; TETRACYCLINE
- Publication
Nature Medicine, 2007, Vol 13, Issue 4, p498
- ISSN
1078-8956
- Publication type
Article
- DOI
10.1038/nm1497