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- Title
Effects of the Absorption Behaviour of ZnO Nanoparticles on Cytotoxicity Measurements.
- Authors
Najim, Nigar; Rusdi, Roshidah; Hamzah, Ahmad Sazali; Shaameri, Zurina; Mat Zain, Mazatulikhma; Kamarulzaman, Norlida
- Abstract
ZnO absorbs certain wavelengths of light and this behavior is more pronounced for nanoparticles of ZnO. As many toxicity measurements rely on measuring light transmission in cell lines, it is essential to determine how far this light absorption influences experimental toxicity measurements.Themain objective was to study the ZnO absorption and how this influenced the cytotoxicity measurements. The cytotoxicity of differently sized ZnO nanoparticles in normal and cancer cell lines derived from lung tissue (Hs888Lu), neuron-phenotypic cells (SH-SY5Y), neuroblastoma (SH-SY5Y), human histiocytic lymphoma (U937), and lung cancer (A549) was investigated. Our results demonstrate that the presence of ZnO affected the cytotoxicity measurements due to the absorption characteristic of ZnOnanoparticles.The data revealed that the ZnOnanoparticleswith an average particle size of around 85.7nm and 190nm showed cytotoxicity towards U937, SH-SY5Y, differentiated SH-SY5Y, and Hs888Lu cell lines. No effect on the A549 cells was observed. It was also found that the cytotoxicity of ZnO was particle size, concentration, and time dependent.These studies are the first to quantify the influence of ZnO nanoparticles on cytotoxicity assays. Corrections for absorption effects were carried out which gave an accurate estimation of the concentrations that produce the cytotoxic effects.
- Subjects
ZINC oxide; CELL-mediated cytotoxicity; NANOPARTICLES; ABSORPTION; WAVELENGTHS; PHENOTYPES; NEUROBLASTOMA
- Publication
Journal of Nanomaterials, 2014, p1
- ISSN
1687-4110
- Publication type
Article
- DOI
10.1155/2014/694737