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- Title
Seungmagalgeun-Tang, a Traditional Herbal Formula, Alleviates Skin Inflammation and Depression-Like Behavior in Atopic Dermatitis Mice under Sleep Deprivation Conditions.
- Authors
Thi Huong Nguyen, Ly; Choi, Min-Jin; Shin, Heung-Mook; Yang, In-Jun
- Abstract
Atopic dermatitis (AD) is a common inflammatory skin disease, which can be worsened under sleep deprivation (SD) conditions. This study investigated the efficacy and the mechanism of action of the traditional herbal formula Seungmagalgeun-tang (SMGGT) on the inflammation and behavioral changes in a mouse model of AD exposed to SD. SMGGT decreased levels of IgE, TNF-α, IL-4, IL-13, and mast cell infiltration and reduced the expression of CD3 in the mouse skin. SMGGT also reversed the SD-induced increase in corticosterone and decrease in melatonin level. Furthermore, SMGGT reduced the immobility time in the tail suspension test significantly. HaCaT cells and HMC-1 cells were used to investigate the effects of SMGGT on cell signaling pathways. In TNF-α/IFN-γ (TI) treated HaCaT cells, SMGGT reduced production of TARC/CCL17 and MDC/CCL22 and suppressed the p38 MAPK, STAT1, and NF-κB pathways. In substance P (SP)/CRH-stimulated HMC-1 cells, SMGGT decreased VEGF production and inhibited ERK phosphorylation. Network pharmacology and molecular docking analysis revealed that puerarin and paeoniflorin might contribute to the effects of SMGGT by targeting several AD-related molecules and pathways. Puerarin and paeoniflorin exerted anti-inflammatory effects by decreasing production of MDC/CCL22 and IL-6 in TI-treated HaCaT cells and VEGF production in SP/CRH-stimulated HMC-1 cells. This study suggests that SMGGT with puerarin and paeoniflorin as main bioactive components alleviates skin inflammation and depression-like behavior in a sleep-deprived mouse model of AD.
- Subjects
PHYTOTHERAPY; PREVENTION of mental depression; INFLAMMATION prevention; THERAPEUTIC use of plant extracts; DRUG efficacy; BIOLOGICAL models; INTERLEUKINS; HERBAL medicine; IMMUNOGLOBULINS; ANIMAL experimentation; ANTI-inflammatory agents; SLEEP disorders; GENE expression; MELATONIN; CELLULAR signal transduction; ATOPIC dermatitis; SLEEP deprivation; TUMOR necrosis factors; MAST cells; PLANT extracts; PHARMACEUTICAL chemistry; COMPUTER-assisted molecular modeling; BEHAVIOR modification; MICE; PHOSPHORYLATION
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2022, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2022/1307173